A Comparative Study on Microbial Growth and Substrate Utilization Kinetics
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IJPT | July-2013 | Vol. 5 | Issue No.2 | 5567-5573 Page 5567 ISSN: 0975-766X CODEN: IJPTFI Available Online through Research Article www.ijptonline.com A COMPARATIVE STUDY ON MICROBIAL GROWTH AND SUBSTRATE UTILIZATION KINETICS Merina Paul Das*, Santosh Kumar Department of Industrial Biotechnology, Bharath University, Chennai, India. Email: [email protected] Received on 02-07-2013 Accepted on 17-07-2013 Abstract Microbial growth and substrate utilization kinetics have been studied by many researches time to time in order to estimate the specificity of the particular microbe as potential bioremedial tool. In this present study, bacterial strains were screened and identified for low density polyethylene (LDPE) degradation. Two of the bacterial isolates genetically identified as Bacillus amyloliquefaciens BSM-1 and Bacillus amyloliquefaciens BSM-2 exhibited significant potential for LDPE degradation of 11% and 16% respectively after 60 d of incubation. This study was carried out to understand and reveal that the variation in specific growth rate and doubling time on LDPE supplemented media compared to that of nutrient broth. The specific growth rate of BSM-1 was 8.3X10 -3 h -1 and generation time 4.66 h while for BSM-2 was 0.011 h -1 with generation time 5.25 h for supplemented media. In case of nutrient broth, doubling time for BSM-1 and BSM-2 was 1.8 h and 2.4 h respectively whereas specific growth rate for BSM-1 was 0.44 h -1 and for BSM-2 was 0.625 h -1 .
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